Pcr-based Specific Detection of Ralstonia Solanacearum in Soil, Seed and Infected Plant Material
نویسنده
چکیده
DNA-based methods have provided powerful tools to identify and detect microorganisms with high sensitivity and specificity. PCR assay amplifies the DNA of bacterial pathogens, targeting the species-specific sequences in their genome. In the present study an efficient DNA isolation protocol and PCR based detection of bacterial wilt pathogen in soil, seed and infected plant materials has been described. The specific primers 759f/760r was successfully used to detect Ralstonia solanacearum from different sources and predicted 281-bp DNA fragment was obtained. In conclusion, the PCR-based detection method using R. solanacearum specific primer offers a rapid and sensitive method for unambiguous detection of this pathogen in soil, seed and infected tomato plant materials.
منابع مشابه
Integrated approach for detection of nonculturable cells of Ralstonia solanacearum in asymptomatic Pelargonium spp. cuttings.
Ralstonia solanacearum (biovar 2, race 3) is a soil and water-borne pathogen that causes serious diseases in several solanaceous hosts. It can also infect geranium plants, posing an important threat to their culture when latently infected cuttings are imported from countries where the pathogen is endemic. R. solanacearum can be present in very low numbers in asymptomatic geranium cuttings, and/...
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